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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/11/2016 |
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Data da última atualização: |
07/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIGLIOTI, R.; OLIVEIRA, H. N. de; SANTANA, C. H.; IBELLI, A. M. G.; NEO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. |
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Afiliação: |
Rodrigo Giglioti, UNESP; Henrique Nunes de Oliveira, UNESP; Clarissa Helena Santana, UNESP; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Thalita Athiê Néo, UFSCAR; Talita Barban Bilhassi, UNESP; MARCIO DIAS RABELO, CPPSE; Rosângela Zacarias Machado, UNESP; LUCIANA GATTO BRITO, CPAF-Rondonia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 7, n. 5, p. 657-662, jul. 2016. |
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ISSN: |
1877-959X |
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Idioma: |
Inglês |
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Conteúdo: |
The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. MenosThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus a... Mostrar Tudo |
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Palavras-Chave: |
QPCR; R microplus; Resistance. |
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Thesagro: |
Babesia Bovis; Bovino. |
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Thesaurus Nal: |
babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03252naa a2200313 a 4500
001 2057560
005 2018-11-07
008 2016 bl uuuu u00u1 u #d
022 $a1877-959X
100 1 $aGIGLIOTI, R.
245 $aBabesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil.$h[electronic resource]
260 $c2016
520 $aThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal.
650 $ababesiosis
650 $aBabesia Bovis
650 $aBovino
653 $aQPCR
653 $aR microplus
653 $aResistance
700 1 $aOLIVEIRA, H. N. de
700 1 $aSANTANA, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aNEO, T. A.
700 1 $aBILHASSI, T. B.
700 1 $aRABELO, M. D.
700 1 $aMACHADO, R. Z.
700 1 $aBRITO, L. G.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 7, n. 5, p. 657-662, jul. 2016.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 121 | |
| 62. |  | VIANA, M. B.; DAMS, R. I.; PINHEIRO, B. M.; LEITAO, R. C.; SANTAELLA, S. T.; SANTOS, A. B. DOS. The source of inoculum and the method of methanogenesis inhibition can affect biological hydrogen production from crude glycerol. Bioenergy Research, Berlin, v. 12, n. 3, p. 733-742, Sept. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 67. | | USBERTI FILHO, J. A.; AZZINI, L. E.; SOAVE, J.; VITTI, P.; LEITAO, R. F. de F. PIZZINATTO, A.; VILLELA, O. V.; GALLO, P. B. Características agronômicas e de qualidade de produto de cultivares de arroz de sequeiro e irrigado. Pesquisa Agropecuaria Brasileira, Brasilia, v.21, n.12, p.1283-1296, dez. 1986.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 68. | | ROSA, M. F.; LEITÃO, R. C.; SANTAELLA, S. T.; MATTOS, A. L. A.; CRISÓSTOMO, L. A.; FIGUEIREDO, M. C. B.; BEZERRA, F. C.; VERAS, L. G.; CORREIA, D. Aproveitamento integral da casca do coco verde. In: CONFERÊNCIA INTERNACIONAL EM SANEAMENTO SUSTENTÁVEL: SEGURANÇA ALIMENTAR E HÍDRICA PARA AMÉRICA LATINA, 2007, Fortaleza. Anais... Fortaleza: Ecosanlac, 2007. 4 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 69. | | VASCONCELOS, E. A. F. DE; SANTAELLA, S. T.; VIANA, M. B.; SANTOS, A. B. DOS; PINHEIRO, G. C.; LEITAO, R. C. Composition and ecology of bacterial and archaeal communities in anaerobic reactor fed with residual glycerol. Anaerobe, London, v. 59, p. 145-153, Oct. 2019| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 70. | | NASCIMENTO, D. M. do; ALMEIDA, J. S.; VALE, M. do S.; LEITAO, R. C.; FIGUEIREDO, M. C. B. de; MORAIS, J. P. S.; ROSA, M. de F. A comprehensive approach for obtaining cellulose nanocrystal from coconut fiber. Part I: Proposition of technological pathways. Industrial Crops and Products, v. 93, p. 66-75, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 71. | | SILVA, A. P. F. de S.; COSTA, M. C.; LOPES, A. C.; ABDALA NETO, E. F.; LEITAO, R. C.; MOTA, C. R.; SANTOS, A. B. dos. Comparison of pretreatment methods for total lipids extraction from mixed microalgae. Renewable Energy, v. 63, p. 762-766, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 73. | | MORAIS, N. W. S.; COELHO, M. M. H.; FERREIRA, T. G. T.; PEREIRA, E. L.; LEITAO, R. C.; SANTOS, A. B. DOS. A kinetic study on carboxylic acids production using bovine slaughterhouse wastewater: a promising substrate for resource recovery in biotechnological processes. Bioprocess and Biosystems Engineering, v. 44, n. 2, p. 271-282, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 75. | | NASCIMENTO, D. M. do; NUNES, Y. N.; ALMEIDA, J. de; LEITAO, R. C.; FEITOSA, J. P. A.; DUFRESNE, A.; ROSA, M. de F. Development of an integrated process to produce CNFs and lignin and its potential applications for agrochemical delivery. Cellulose, v. 28, p. 10891-10904, 2021.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 76. | | DAMS, R. I.; VIANA, M. B. V.; GUILHERME, A. A.; SILVA, C. M.; SANTOS, A. B. dos; ANGENENT, L. T.; SANTAELLA, S. T.; LEITAO, R. C. Production of medium-chain carboxylic acids by anaerobic fermentation of glycerol using a bioaugmented open culture. Biomass and Bioenergy, v. 118, p. 1-7, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 78. | | LEITAO, R. C.; GEHRING, T. A.; CAVALCANTE, W. de A.; FREITAS, I. B. F.; COLARES, A. S.; HAANDEL, A. C. van; ANGENENT, L. T.; SANTAELLA, S. T. Produção biológica de ácido caproico em um reator em escala de laboratório. Fortaleza: Embrapa Agroindústria Tropical, 2017. 6 p. (Embrapa Agroindústria Tropical. Comunicado técnico, 229).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 79. | | CORDEIRO, E. M. S.; SILVA NETO, A. A. da; PINHEIRO, F. G. C.; LEITAO, R. C.; CASSALES, A. R.; ARAÚJO, M. L. H.; BENEVIDES, N. M. B. Sacarificação por hidrólise ácida do subproduto da extração de hidrocolóides a macroalga marinha Solieria filiformis. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 20., 2015, Fortaleza. Anais... Fortaleza: ABEQ, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 80. | | ROSA, M. de F.; SOUZA FILHO, M. DE S.; FIGUEIREDO, M. C. B.; MORAIS, J. P. S.; SANTAELLA, S. T.; LEITÃO, R. C. Reaproveitamento de resíduos de plantas da cadeia do biodiesel. In: LUCENA, E. M. P. de; AMORIM, A. V. (Org.). Botânica e desenvolvimento sustentável: palestras magistrais, simpósios e mesas-redondas do 62 Congresso Nacional de Botânica. Fortaleza: EdUECE, 2011. 371 p. p. 161-164| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 121 | |
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